Insulin infusion suppresses endotoxin-induced oxidative, nitrative and inflammatory stress in normal human subjects

      Our work over the past few years has demonstrated that insulin exerts anti-inflammatory and cardio-protective effects. We have now hypothesized that insulin reduces the magnitude of oxidative, nitrative and inflammatory response induced by endotoxin (LPS). Nine normal subjects were injected with 2 ng/Kg of LPS prepared from E. coli intravenously. Ten others were infused with insulin (2U/h) for 6 h in addition to the LPS injection along with 100 ml/hr of 5% dextrose was co-infused with insulin to maintain normoglycemia. LPS injection induced an increase in body temperature, pulse rate, body aches and headache. The subjects also exhibited a rapid increase in plasma concentrations of nitric oxide metabolites (NOM), nitrite and nitrate, TBARS, increase in ROS generation by PMNL and marked increases in plasma FFA, MIF, TNFa and IL-6 concentrations (Table1)The coinfusion of insulin led to significant reduction in body temperature (100.3 vs. 101.3 F, p< 0.005) and headache, a total elimination of the increase in NOM, FFA, TBARS and a significant reduction in ROS generation by PMNL and plasma MIF concentrations (Table 1). However, insulin did not affect TNFa and IL-6. Thus, insulin reduced clinical symptoms, oxidative, nitrative and inflammatory tress as induced by LPS. Insulin is a potential rational anti-inflammatory therapy for endotoxemia.
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