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In Vivo Type 2 Diabetes and Wound-Healing Effects of Antioxidant Gold Nanoparticles Synthesized Using the Insulin Plant Chamaecostus cuspidatus in Albino Rats

      Abstract

      Background

      Gold nanoparticles are known for their many applications in the fields of therapeutics and diagnosis.

      Methods

      This article focuses mainly on the green method of synthesizing gold nanoparticles by using the leaf powder extract of the insulin plant Chamaecostus cuspidatus and on the characterization of developed plant-mediated synthesis of gold nanoparticles. Furthermore, we investigated the free-radical scavenging activity of green-synthesized gold nanoparticles.

      Results

      The free radicals were exhibited in a dose-dependent manner. The 50% inhibition of free radicals by gold nanoparticles showed that it was similar to that of the standard inhibition. Toxicity studies generally examine changes in blood serum chemistry and cell populations in tissue morphology through histologic analysis without inducing any lethal effects in the mouse model, thereby accomplishing sustained control over the progression of diabetes mellitus, which plays a leading role in vascular complications in patients. The treatment by gold nanoparticles of the mice with diabetes for a period of 21 days restored their blood glucose, glycogen and insulin levels.

      Conclusions

      The use of gold nanoparticles as antidiabetes materials has been achieved. Further studies are required before gold nanoparticle–based drugs are more widely used.

      Résumé

      Contexte

      Les nanoparticules d'or sont connues pour leurs nombreuses applications dans les domaines de la thérapeutique et du diagnostic.

      Méthodes

      Le présent article porte principalement sur la méthode de synthèse verte des nanoparticules d'or à partir de la poudre d'extrait de feuilles de la plante d'insuline Chamaecostus cuspidatus et sur la caractérisation de la synthèse médiée par la plante des nanoparticules d'or. De plus, nous avons examiné l'activité de piégeage des radicaux libres des nanoparticules d'or synthétisées par la méthode verte.

      Résultats

      Les radicaux libres ont été exposés de façon proportionnelle à la dose. L'inhibition de 50 % des radicaux libres par les nanoparticules d'or a montré un résultat similaire avec l'inhibition habituelle. Les études sur la toxicité visent généralement à examiner les modifications de la chimie du sérum sanguin et les populations cellulaires dans la morphologie tissulaire par l'étude histologique sans réduire les effets létaux dans le modèle de souris, mais pour maîtriser de manière prolongée l'évolution du diabète sucré, qui joue un rôle prépondérant dans la survenue des complications vasculaires chez les patients. Le traitement des souris diabétiques par nanoparticules d'or durant 21 jours rétablit leurs concentrations de glucose dans le sang, en glycogène et d'insuline.

      Conclusions

      L'utilisation des nanoparticules d'or comme matériaux antidiabétiques a été démontrée; les médicaments à base de nanoparticules d'or seront davantage utilisés s'ils font l'objet d'autres études.

      Keywords

      Mots clés

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